cd11b-pe cy7 m1 Search Results


cd11b pe cy7  (Miltenyi Biotec)
98
Miltenyi Biotec cd11b pe cy7
Cd11b Pe Cy7, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat-anti-mouse cd11b pe-cy7 m1/70  (Becton Dickinson)
90
Becton Dickinson rat-anti-mouse cd11b pe-cy7 m1/70
Rat Anti Mouse Cd11b Pe Cy7 M1/70, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe-cy7-anti-mouse-cd11b m1/70  (Thermo Fisher)
90
Thermo Fisher pe-cy7-anti-mouse-cd11b m1/70
Pe Cy7 Anti Mouse Cd11b M1/70, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe-cy7-conjugated cd11b antibody  (Thermo Fisher)
90
Thermo Fisher pe-cy7-conjugated cd11b antibody
Pe Cy7 Conjugated Cd11b Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe cy7 cd11b  (Thermo Fisher)
86
Thermo Fisher pe cy7 cd11b
a , Experimental protocol for the development of gut allergic inflammation and systemic anaphylaxis. Allergic BALB/c WT and littermate IgE KO mice were sensitized with OVA/alum whereas control mice received alum alone. All mice were orally challenged with OVA. b , Rectal temperature over time (left) and maximum temperature variation (right) after systemic OVA challenge on d1 (n = 4 WT control and n = 5 allergic, IgE KO control and allergic). c , OVA-specific IgE (left) and IgG1 (right) serum antibodies after five oral challenges with OVA (n = 13-17 per group L, n = 5-9 per group R). d , Gastrointestinal transit time was determined on day 31 following OVA challenge (n = 7 WT controls, 10 WT allergic, 10 IgE KO controls, 8 IgE KO allergic). e , Systemic levels of corticosterone 1h after the fifth OVA challenge in control and OVA/alum sensitized mice (n = 14 WT controls and allergic, 5 IgE KO controls and allergic). f-g , Flow cytometry analysis of isolated small intestinal cells from the epithelial layer (left) and lamina propria (centre and right) (n = 8 WT control and allergic, 6 IgE KO control, 5 IgE KO allergic, f ) (n = 11 WT control, 13 WT allergic, 10 IgE KO control, 7 IgE KO allergic, g ). h , Flow cytometry analysis of enteric mast cells (CD45+ CD19- CD3- <t>CD11b-</t> CD117int FcεRI+ cells) from the small intestine of OVA/alum sensitized BALB/c WT mice after OVA challenges. Controls were sensitized with alum alone and challenged with oral OVA. Graphs show mean ± s.e.m. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. Two-tailed Mann-Whitney test. Each panel is representative of at least two independent experiments. a , Created with BioRender.com .
Pe Cy7 Cd11b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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antibody rat anti-cd11b pe-cy7 (clone: m1/70)  (Thermo Fisher)
90
Thermo Fisher antibody rat anti-cd11b pe-cy7 (clone: m1/70)
Reagents table
Antibody Rat Anti Cd11b Pe Cy7 (Clone: M1/70), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody rat anti-cd11b pe-cy7 (clone: m1/70)/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
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cd11b pe-cy7 (m1/70  (Thermo Fisher)
90
Thermo Fisher cd11b pe-cy7 (m1/70
Reagents table
Cd11b Pe Cy7 (M1/70, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd11b-pe-cy7  (Thermo Fisher)
90
Thermo Fisher cd11b-pe-cy7
Reagents table
Cd11b Pe Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat anti-human cd47 b6h12  (Becton Dickinson)
90
Becton Dickinson rat anti-human cd47 b6h12
(A) Marrow macrophages were engineered and mixed with streptavidin beads of 900 nm to 6.7 μm diameter or else with disaggregated tdTom A549 tumors. Beads are opsonized with anti-streptavidin except for a non-targeted control in which SIRPα-inhibited macrophages were incubated with 2.1 μm lacking anti-streptavidin. These were not engulfed. After pre-incubation of the mixtures for up to 150 min, mixtures were plated for 24 hr on top of 5-μm pore transwells. Cells collected from transwell tops and bottoms were stained for <t>CD11b+,F4/80+</t> and analyzed by flow cytometry for engulfment (STAR Methods).
Rat Anti Human Cd47 B6h12, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd11b (pe-cy7, m1/70  (Thermo Fisher)
90
Thermo Fisher cd11b (pe-cy7, m1/70
(A) Marrow macrophages were engineered and mixed with streptavidin beads of 900 nm to 6.7 μm diameter or else with disaggregated tdTom A549 tumors. Beads are opsonized with anti-streptavidin except for a non-targeted control in which SIRPα-inhibited macrophages were incubated with 2.1 μm lacking anti-streptavidin. These were not engulfed. After pre-incubation of the mixtures for up to 150 min, mixtures were plated for 24 hr on top of 5-μm pore transwells. Cells collected from transwell tops and bottoms were stained for <t>CD11b+,F4/80+</t> and analyzed by flow cytometry for engulfment (STAR Methods).
Cd11b (Pe Cy7, M1/70, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd11b-pe/cy7  (Thermo Fisher)
90
Thermo Fisher cd11b-pe/cy7
(A) Marrow macrophages were engineered and mixed with streptavidin beads of 900 nm to 6.7 μm diameter or else with disaggregated tdTom A549 tumors. Beads are opsonized with anti-streptavidin except for a non-targeted control in which SIRPα-inhibited macrophages were incubated with 2.1 μm lacking anti-streptavidin. These were not engulfed. After pre-incubation of the mixtures for up to 150 min, mixtures were plated for 24 hr on top of 5-μm pore transwells. Cells collected from transwell tops and bottoms were stained for <t>CD11b+,F4/80+</t> and analyzed by flow cytometry for engulfment (STAR Methods).
Cd11b Pe/Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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135523 cd11b pe cy7  (Thermo Fisher)
86
Thermo Fisher 135523 cd11b pe cy7
(A) Marrow macrophages were engineered and mixed with streptavidin beads of 900 nm to 6.7 μm diameter or else with disaggregated tdTom A549 tumors. Beads are opsonized with anti-streptavidin except for a non-targeted control in which SIRPα-inhibited macrophages were incubated with 2.1 μm lacking anti-streptavidin. These were not engulfed. After pre-incubation of the mixtures for up to 150 min, mixtures were plated for 24 hr on top of 5-μm pore transwells. Cells collected from transwell tops and bottoms were stained for <t>CD11b+,F4/80+</t> and analyzed by flow cytometry for engulfment (STAR Methods).
135523 Cd11b Pe Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a , Experimental protocol for the development of gut allergic inflammation and systemic anaphylaxis. Allergic BALB/c WT and littermate IgE KO mice were sensitized with OVA/alum whereas control mice received alum alone. All mice were orally challenged with OVA. b , Rectal temperature over time (left) and maximum temperature variation (right) after systemic OVA challenge on d1 (n = 4 WT control and n = 5 allergic, IgE KO control and allergic). c , OVA-specific IgE (left) and IgG1 (right) serum antibodies after five oral challenges with OVA (n = 13-17 per group L, n = 5-9 per group R). d , Gastrointestinal transit time was determined on day 31 following OVA challenge (n = 7 WT controls, 10 WT allergic, 10 IgE KO controls, 8 IgE KO allergic). e , Systemic levels of corticosterone 1h after the fifth OVA challenge in control and OVA/alum sensitized mice (n = 14 WT controls and allergic, 5 IgE KO controls and allergic). f-g , Flow cytometry analysis of isolated small intestinal cells from the epithelial layer (left) and lamina propria (centre and right) (n = 8 WT control and allergic, 6 IgE KO control, 5 IgE KO allergic, f ) (n = 11 WT control, 13 WT allergic, 10 IgE KO control, 7 IgE KO allergic, g ). h , Flow cytometry analysis of enteric mast cells (CD45+ CD19- CD3- CD11b- CD117int FcεRI+ cells) from the small intestine of OVA/alum sensitized BALB/c WT mice after OVA challenges. Controls were sensitized with alum alone and challenged with oral OVA. Graphs show mean ± s.e.m. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. Two-tailed Mann-Whitney test. Each panel is representative of at least two independent experiments. a , Created with BioRender.com .

Journal: Nature

Article Title: Immune sensing of food allergens promotes avoidance behaviour

doi: 10.1038/s41586-023-06362-4

Figure Lengend Snippet: a , Experimental protocol for the development of gut allergic inflammation and systemic anaphylaxis. Allergic BALB/c WT and littermate IgE KO mice were sensitized with OVA/alum whereas control mice received alum alone. All mice were orally challenged with OVA. b , Rectal temperature over time (left) and maximum temperature variation (right) after systemic OVA challenge on d1 (n = 4 WT control and n = 5 allergic, IgE KO control and allergic). c , OVA-specific IgE (left) and IgG1 (right) serum antibodies after five oral challenges with OVA (n = 13-17 per group L, n = 5-9 per group R). d , Gastrointestinal transit time was determined on day 31 following OVA challenge (n = 7 WT controls, 10 WT allergic, 10 IgE KO controls, 8 IgE KO allergic). e , Systemic levels of corticosterone 1h after the fifth OVA challenge in control and OVA/alum sensitized mice (n = 14 WT controls and allergic, 5 IgE KO controls and allergic). f-g , Flow cytometry analysis of isolated small intestinal cells from the epithelial layer (left) and lamina propria (centre and right) (n = 8 WT control and allergic, 6 IgE KO control, 5 IgE KO allergic, f ) (n = 11 WT control, 13 WT allergic, 10 IgE KO control, 7 IgE KO allergic, g ). h , Flow cytometry analysis of enteric mast cells (CD45+ CD19- CD3- CD11b- CD117int FcεRI+ cells) from the small intestine of OVA/alum sensitized BALB/c WT mice after OVA challenges. Controls were sensitized with alum alone and challenged with oral OVA. Graphs show mean ± s.e.m. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. Two-tailed Mann-Whitney test. Each panel is representative of at least two independent experiments. a , Created with BioRender.com .

Article Snippet: The following antibodies were used at a concentration of 1 mg ml −1 except where otherwise indicated: APC–Cy7–CD117 (clone 2B8; Biolegend no. 105826), APC/eFluor780–MHCII (clone M5/114.15.2; eBioscience 47-5321-82), APC/eFluor780–CD19 (clone eBio1D3; eBioscience 47-0193-82), APC/eFluor780–CD4 (clone RM4-5; Invitrogen 47-0042-82), PE–FCER1 (clone MAR-1; eBioscience 12-5898-82), PE–SiglecF (clone E50-2440; BD Pharmingen no. 552126), PE–Gata3 (clone TWAJ; eBioscience no. 12-9966-42), eFluor450-CD45 (clone 30-F11; eBioscience no. 48-0451-82), eFluor450–FCER1 (clone MAR-1; eBioscience no. 48-5898-82), APC–CD11b (clone M1/70; eBioscience no. 17-0112-82), APC–Ly6c (clone HK1.4; eBioscience no. 17-5932-82), APC–TCRb (clone H57-597; Biolegend no. 109212), APC–SA (eBioscience no. 17-4317-82), APC–MHCII (clone M5/114.15.2; eBioscience no. 17-5321-82), Alexa700-CD3 (clone 17A2; Biolegend no. 100216), Alexa700–CD45 (clone 30-F11; Biolegend no. 103128), Alexa700–CD19 (clone 6D5; Biolegend no. B189284), PE/Cy–CD3e (clone 145-SC11, eBioscience no. 25-0031-82), PE/Cy7–Ly6G (clone RB6-8C5; eBioscience no. 25-5931-82), PE/Cy7–CD117 (clone 2B8; eBioscience no. 25-1171-82), PE/Cy7–Tbet (clone eBio4B10; eBioscience no. 25-5825-82), PE/Cy7–CD4 (clone GK1.5; Biolegend no. 100422), PE/Cy7–CD11b (clone M1/70; eBioscience no. 25-0112-82), PE/Cy7–CD45R (clone RA3-6B2; eBioscience no. 25-0452-82), PE/Cy7–NK1.1 (clone PK136; BD Pharmingen no. 552878), FITC–CD11c (clone N418; eBioscience no. 11-0114-85), FITC–IgE (clone R35-72; BD Pharmingen no. 553415), FITC–CD11b (clone M1/70; eBioscience no. 11-0112-85), FITC–CD19 (clone eBio1D3; eBioscience no. 11-0193-82), FITC–Gr1 (Ly6G/Ly6c; clone RB6-8C5; Biolegend no. 108406), FITC–NK1.1 (clone PK136; Biolegend no. 108706), FITC–Ter119 (clone Ly76; Biolegend no. 116206), FITC–CD49b (clone DX5; Biolegend no. 108906), FITC–Lin (clones 145-2C11, RB6-8C5, RA3-6B2, Ter119, M1/70; Biolegend no. 133301), FITC–MHCII (clone M5/114.15.2; eBioscience no. 11-5321-82), biotin–IgE (clone R35-72; BD Pharmingen no. 553414), BV711–F4/80 (clone T45-2342; BD Horizon no. 565612), BV421–CD11b (clone M1/70; BD Horizon no. 562605), BV421–RORgt (clone Q31-378; BD Horizon no. 562894), BUV395–CD45 (clone 30-F11; BD Horizon no. 564279), BUV737–CD90.2 (clone 53-2.1; BD Bioscience no. 741701) and PECy5.5–Foxp3 (clone FJK-16s; eBioscience no. 35-5773-82).

Techniques: Flow Cytometry, Isolation, Two Tailed Test, MANN-WHITNEY

Reagents table

Journal: Nature Communications

Article Title: RIG-I-like receptors direct inflammatory macrophage polarization against West Nile virus infection

doi: 10.1038/s41467-019-11250-5

Figure Lengend Snippet: Reagents table

Article Snippet: Antibody , Rat anti-CD11b PE-Cy7 (clone: M1/70) (1:160 dilution) , Thermo Fisher Scientific , Cat#25-01112-82; lot#E07514-1633; RRID: AB_469588.

Techniques: Virus

(A) Marrow macrophages were engineered and mixed with streptavidin beads of 900 nm to 6.7 μm diameter or else with disaggregated tdTom A549 tumors. Beads are opsonized with anti-streptavidin except for a non-targeted control in which SIRPα-inhibited macrophages were incubated with 2.1 μm lacking anti-streptavidin. These were not engulfed. After pre-incubation of the mixtures for up to 150 min, mixtures were plated for 24 hr on top of 5-μm pore transwells. Cells collected from transwell tops and bottoms were stained for CD11b+,F4/80+ and analyzed by flow cytometry for engulfment (STAR Methods).

Journal: Current biology : CB

Article Title: SIRPA-Inhibited, Marrow-Derived Macrophages Engorge, Accumulate, and Differentiate in Antibody-Targeted Regression of Solid Tumors

doi: 10.1016/j.cub.2017.06.005

Figure Lengend Snippet: (A) Marrow macrophages were engineered and mixed with streptavidin beads of 900 nm to 6.7 μm diameter or else with disaggregated tdTom A549 tumors. Beads are opsonized with anti-streptavidin except for a non-targeted control in which SIRPα-inhibited macrophages were incubated with 2.1 μm lacking anti-streptavidin. These were not engulfed. After pre-incubation of the mixtures for up to 150 min, mixtures were plated for 24 hr on top of 5-μm pore transwells. Cells collected from transwell tops and bottoms were stained for CD11b+,F4/80+ and analyzed by flow cytometry for engulfment (STAR Methods).

Article Snippet: REAGENT OR RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human SIRPα (clone SE7C2) Santa Cruz sc-23863 Rat anti-mouse SIRPα (clone P84) BD Biosciences 552371 Rabbit anti-human RBCs (polyclonal) Rockland 109-4139 Anti-human CD41-FITC (HIP8) Biolegend 303703 Anti-human CD47-FITC (Clone CC2C6) Biolegend 323106 Rat Anti-human CD47 (Clone B6H12) BD Biosciences 556044 Anti-mouse CD11b-PE/Cy7 (Clone M1/70) Biolegend 101215 Anti-mouse F4/80-APC/Cy7 (clone BM8) Biolegend 123113 Donkey Anti-rabbit AF488 ThermoFisher A-21206 Donkey Anti-rabbit AF647 ThermoFisher A-31573 IgG from rabbit serum Sigma I5006-10MG Mouse anti-human Mucin (Clone S.854.6) ThermoFisher MA5-15131 Cetuximab InVivogen hegfr-mab1 Goat anti-rabbit AF700 ThermoFisher A-21038 Goat anti-rat AF647 ThermoFisher A-21247 Anti-mouse Ly-6G (Gr-1) APC (Clone RB6-8C5) Biolegend 108411 Anti-mouse CD135-BV421 (Clone A2F10.1) Biosciences 562898 Anti-mouse SIRPα-FITC (Clone P84) Biosciences 144005 Anti-human CD14-BV421 (Clone HCD14) Biolegend 325627 Anti-human CD33-APC (Clone WM53) Biolegend 303407 Anti-human CD66-PerCP/Cy5.5 (Clone G10F5) Biolegend 305107 Biological Samples Human Bone Marrow AllCells ABM001-1 NSG Mice The Jackson Laboratory 5557 Chemicals, Peptides, and Recombinant Proteins 7-Amino-actinomycin D Sigma A9400-1MG Hoechst 33342 ThermoFisher H3570 Phorbol myristate acetate Sigma P8139-1MG CFDA-SE ThermoFisher V12883 RBC Lysis Buffer Sigma R7757 Dispase StemCell Technologies 7913 Collagenase Sigma C0130-100MG DNase I Sigma 11284932001 Anti-streptavidin (polyclonal) Sigma S6390 Critical Commercial Assays F(Ab′)2 Preparation Kit ThermoFisher 44988 SIRPα ShRNA Lentiviral Plasmid Santa Cruz sc-44106-SH Streptavidin Beads ThermoFisher 11205D RNeasy plus Mini Kit QIAGEN 74134 TruSeq Stranded mRNA Library Prep kit Illumina RS-122-2101 Cell Lines Human adenocarcinoma (A549) ATCC CCL-185 Human Monocyte (THP-1) ATCC TIB-202 Primary liver carcinoma (EC4) Chi Van Dang’s Lab N/A Mesenchymal Stem Cells Progeria Research Foundation N/A Human hepatocellular carcinoma (HepG2) ATCC HB-8065 Mouse myoblast (C2C12) ATCC CRL-1772 Software MaxQuant Version 1.5.3.8 Max Planck Institute of Biochemistry http://www.biochem.mpg.de/5111795/maxquant Living Image Perkin Elmer http://www.perkinelmer.com/product/spectrum-200-living-image-v4series-1-128113 ImageJ NIH https://imagej.nih.gov/ij/ Open in a separate window KEY RESOURCES TABLE

Techniques: Incubation, Staining, Flow Cytometry

(A) Sirpa:Cd47 ratios increase in macrophages from microenvironments of increasing stiffness.

Journal: Current biology : CB

Article Title: SIRPA-Inhibited, Marrow-Derived Macrophages Engorge, Accumulate, and Differentiate in Antibody-Targeted Regression of Solid Tumors

doi: 10.1016/j.cub.2017.06.005

Figure Lengend Snippet: (A) Sirpa:Cd47 ratios increase in macrophages from microenvironments of increasing stiffness.

Article Snippet: REAGENT OR RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human SIRPα (clone SE7C2) Santa Cruz sc-23863 Rat anti-mouse SIRPα (clone P84) BD Biosciences 552371 Rabbit anti-human RBCs (polyclonal) Rockland 109-4139 Anti-human CD41-FITC (HIP8) Biolegend 303703 Anti-human CD47-FITC (Clone CC2C6) Biolegend 323106 Rat Anti-human CD47 (Clone B6H12) BD Biosciences 556044 Anti-mouse CD11b-PE/Cy7 (Clone M1/70) Biolegend 101215 Anti-mouse F4/80-APC/Cy7 (clone BM8) Biolegend 123113 Donkey Anti-rabbit AF488 ThermoFisher A-21206 Donkey Anti-rabbit AF647 ThermoFisher A-31573 IgG from rabbit serum Sigma I5006-10MG Mouse anti-human Mucin (Clone S.854.6) ThermoFisher MA5-15131 Cetuximab InVivogen hegfr-mab1 Goat anti-rabbit AF700 ThermoFisher A-21038 Goat anti-rat AF647 ThermoFisher A-21247 Anti-mouse Ly-6G (Gr-1) APC (Clone RB6-8C5) Biolegend 108411 Anti-mouse CD135-BV421 (Clone A2F10.1) Biosciences 562898 Anti-mouse SIRPα-FITC (Clone P84) Biosciences 144005 Anti-human CD14-BV421 (Clone HCD14) Biolegend 325627 Anti-human CD33-APC (Clone WM53) Biolegend 303407 Anti-human CD66-PerCP/Cy5.5 (Clone G10F5) Biolegend 305107 Biological Samples Human Bone Marrow AllCells ABM001-1 NSG Mice The Jackson Laboratory 5557 Chemicals, Peptides, and Recombinant Proteins 7-Amino-actinomycin D Sigma A9400-1MG Hoechst 33342 ThermoFisher H3570 Phorbol myristate acetate Sigma P8139-1MG CFDA-SE ThermoFisher V12883 RBC Lysis Buffer Sigma R7757 Dispase StemCell Technologies 7913 Collagenase Sigma C0130-100MG DNase I Sigma 11284932001 Anti-streptavidin (polyclonal) Sigma S6390 Critical Commercial Assays F(Ab′)2 Preparation Kit ThermoFisher 44988 SIRPα ShRNA Lentiviral Plasmid Santa Cruz sc-44106-SH Streptavidin Beads ThermoFisher 11205D RNeasy plus Mini Kit QIAGEN 74134 TruSeq Stranded mRNA Library Prep kit Illumina RS-122-2101 Cell Lines Human adenocarcinoma (A549) ATCC CCL-185 Human Monocyte (THP-1) ATCC TIB-202 Primary liver carcinoma (EC4) Chi Van Dang’s Lab N/A Mesenchymal Stem Cells Progeria Research Foundation N/A Human hepatocellular carcinoma (HepG2) ATCC HB-8065 Mouse myoblast (C2C12) ATCC CRL-1772 Software MaxQuant Version 1.5.3.8 Max Planck Institute of Biochemistry http://www.biochem.mpg.de/5111795/maxquant Living Image Perkin Elmer http://www.perkinelmer.com/product/spectrum-200-living-image-v4series-1-128113 ImageJ NIH https://imagej.nih.gov/ij/ Open in a separate window KEY RESOURCES TABLE

Techniques:

KEY RESOURCES TABLE

Journal: Current biology : CB

Article Title: SIRPA-Inhibited, Marrow-Derived Macrophages Engorge, Accumulate, and Differentiate in Antibody-Targeted Regression of Solid Tumors

doi: 10.1016/j.cub.2017.06.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT OR RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human SIRPα (clone SE7C2) Santa Cruz sc-23863 Rat anti-mouse SIRPα (clone P84) BD Biosciences 552371 Rabbit anti-human RBCs (polyclonal) Rockland 109-4139 Anti-human CD41-FITC (HIP8) Biolegend 303703 Anti-human CD47-FITC (Clone CC2C6) Biolegend 323106 Rat Anti-human CD47 (Clone B6H12) BD Biosciences 556044 Anti-mouse CD11b-PE/Cy7 (Clone M1/70) Biolegend 101215 Anti-mouse F4/80-APC/Cy7 (clone BM8) Biolegend 123113 Donkey Anti-rabbit AF488 ThermoFisher A-21206 Donkey Anti-rabbit AF647 ThermoFisher A-31573 IgG from rabbit serum Sigma I5006-10MG Mouse anti-human Mucin (Clone S.854.6) ThermoFisher MA5-15131 Cetuximab InVivogen hegfr-mab1 Goat anti-rabbit AF700 ThermoFisher A-21038 Goat anti-rat AF647 ThermoFisher A-21247 Anti-mouse Ly-6G (Gr-1) APC (Clone RB6-8C5) Biolegend 108411 Anti-mouse CD135-BV421 (Clone A2F10.1) Biosciences 562898 Anti-mouse SIRPα-FITC (Clone P84) Biosciences 144005 Anti-human CD14-BV421 (Clone HCD14) Biolegend 325627 Anti-human CD33-APC (Clone WM53) Biolegend 303407 Anti-human CD66-PerCP/Cy5.5 (Clone G10F5) Biolegend 305107 Biological Samples Human Bone Marrow AllCells ABM001-1 NSG Mice The Jackson Laboratory 5557 Chemicals, Peptides, and Recombinant Proteins 7-Amino-actinomycin D Sigma A9400-1MG Hoechst 33342 ThermoFisher H3570 Phorbol myristate acetate Sigma P8139-1MG CFDA-SE ThermoFisher V12883 RBC Lysis Buffer Sigma R7757 Dispase StemCell Technologies 7913 Collagenase Sigma C0130-100MG DNase I Sigma 11284932001 Anti-streptavidin (polyclonal) Sigma S6390 Critical Commercial Assays F(Ab′)2 Preparation Kit ThermoFisher 44988 SIRPα ShRNA Lentiviral Plasmid Santa Cruz sc-44106-SH Streptavidin Beads ThermoFisher 11205D RNeasy plus Mini Kit QIAGEN 74134 TruSeq Stranded mRNA Library Prep kit Illumina RS-122-2101 Cell Lines Human adenocarcinoma (A549) ATCC CCL-185 Human Monocyte (THP-1) ATCC TIB-202 Primary liver carcinoma (EC4) Chi Van Dang’s Lab N/A Mesenchymal Stem Cells Progeria Research Foundation N/A Human hepatocellular carcinoma (HepG2) ATCC HB-8065 Mouse myoblast (C2C12) ATCC CRL-1772 Software MaxQuant Version 1.5.3.8 Max Planck Institute of Biochemistry http://www.biochem.mpg.de/5111795/maxquant Living Image Perkin Elmer http://www.perkinelmer.com/product/spectrum-200-living-image-v4series-1-128113 ImageJ NIH https://imagej.nih.gov/ij/ Open in a separate window KEY RESOURCES TABLE

Techniques: Recombinant, Lysis, shRNA, Plasmid Preparation, Software